Testosterone, Total (LC/MS/MS)

Fasting Required
CPT: 84403
Hormones

What This Test Measures

Gold-standard mass spectrometry method for total testosterone; more accurate than immunoassay, especially at low levels.

Preparation

Fasting required (8-12 hours)
Draw in the early morning (7–10 AM) when testosterone is at its daily peak. If on testosterone BHRT injections, draw at trough — just before your next scheduled injection — for the most clinically meaningful result. Quest may report inflated values for women on BHRT; CPL or LabCorp recommended for trending.
💬 Patient Community Notes

TRT TIMING: Draw at TROUGH — just before your next injection — for dose adjustment. LC/MS/MS is the gold standard methodology, more accurate than immunoassay especially for women and low-range values. For women on BHRT, this method avoids the false elevations seen with Quest's immunoassay. The TRT community tracks trough levels to ensure adequate coverage between injections. These notes reflect common practices in TRT/BHRT communities.

Reflects common practices in thyroid, BHRT, and functional medicine communities. This may differ from standard clinical guidelines. Not medical advice — always discuss with your provider.

General guidelines only. Requirements vary by lab and individual. Confirm with your provider before adjusting any medications.

Turnaround Time

Varies by lab

Clinical Notes

Preferred method for women and children; Quest code 15983

ICD-10 Codes

Common diagnosis codes associated with this test. Codes are assigned by your provider.

E28.39Other ovarian dysfunction
E29.1Testicular hypofunction
N91.2Amenorrhea, unspecified
R68.89Other specified general symptoms and signs

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Lab Codes

Proprietary codes used by each lab for this test.

ARUP Laboratories
0081058(order_code)
LabCorp
070001(order)
Quest Diagnostics
15983(order)

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For informational purposes only

This page is for educational and research purposes only and does not constitute medical advice, diagnosis, or treatment recommendations. ICD-10 and CPT codes are assigned by your healthcare provider. Always consult a qualified healthcare provider before ordering any lab test or making decisions about your health.