Vitamin D, 25-OH (Total)
What This Test Measures
Measures the storage form of Vitamin D; the best overall indicator of vitamin D status.
Preparation
TIMING: No need to fast, but the functional medicine community recommends stopping vitamin D supplements for 24–48 hours before the draw to avoid an artificial spike from the most recent dose. After starting a loading dose protocol, wait 2–3 months before retesting — it takes time for levels to stabilize. The functional medicine community targets 50–80 ng/mL for immune support and autoimmune conditions, significantly above the standard 30 ng/mL floor. For Hashimoto's, Graves', or chronic illness, practitioners often target the higher end. Note: for patients with hyperparathyroidism or kidney disease, discuss targets with your provider.
Reflects common practices in thyroid, BHRT, and functional medicine communities. This may differ from standard clinical guidelines. Not medical advice — always discuss with your provider.
General guidelines only. Requirements vary by lab and individual. Confirm with your provider before adjusting any medications.
Turnaround Time
Varies by lab
Clinical Notes
Most commonly ordered vitamin test; <20 ng/mL deficiency; 20-30 insufficiency; 30-80 optimal
ICD-10 Codes
Common diagnosis codes associated with this test. Codes are assigned by your provider.
Self-Pay Pricing
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Uses LabCorp draw centers · Not available in NY, NJ, RI, HI, ND, or VT · Membership may be required
Uses Quest Diagnostics network; generally lowest DTC prices; not available in NY, NJ, RI
Uses Labcorp network; known for supplement company; competitive lab test pricing
Uses Quest and Labcorp; strong panel bundling; results in 1-3 days
Uses Quest Diagnostics network; good panel pricing; established DTC provider
Uses both Quest and Labcorp networks; competitive pricing; broad test selection
Uses Quest and Labcorp; no hidden draw or facility fees; moderate pricing
Uses Quest and Labcorp networks; offers both individual tests and discounted panels
Labcorp consumer-direct platform; 2,000+ locations; results in 1-3 days
At-home self-collection. Finger prick or swab — no lab visit required. Results via secure portal within 10 business days.
Primarily uses Labcorp network; popular for hormone panels; higher individual test prices
Quest Diagnostics consumer-direct platform; results in 1-2 days; 2,100+ locations nationwide
Lab Codes
Proprietary codes used by each lab for this test.
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For informational purposes only
This page is for educational and research purposes only and does not constitute medical advice, diagnosis, or treatment recommendations. ICD-10 and CPT codes are assigned by your healthcare provider. Always consult a qualified healthcare provider before ordering any lab test or making decisions about your health.