Cortisol, Salivary (4-point)

CPT: 82530
Hormones

What This Test Measures

Salivary cortisol measured at 4 time points throughout day; assesses diurnal cortisol rhythm.

Preparation

No fasting required
Timing matters for cortisol. AM draws should be between 7–9 AM; PM draws between 3–5 PM. Stress, poor sleep, illness, and recent exercise can all elevate cortisol. Avoid intense exercise before drawing.
💬 Patient Community Notes

PREP: Stop all cortisol-affecting supplements for at least 1 week before testing (hydrocortisone, adrenal adaptogens, licorice root, pregnenolone, etc.). See STTM's list of cortisol-affecting substances. Collect samples at the specified times (waking, midday, afternoon, bedtime). Avoid eating, drinking, or brushing teeth 30–60 minutes before each sample. STTM's optimal pattern: morning at the very top of range, midday in upper quarter, afternoon around mid-range, bedtime at the very bottom. This 4-point test reveals the full daily rhythm that a single AM serum cortisol cannot. These notes reflect common practices in adrenal/functional medicine communities.

Reflects common practices in thyroid, BHRT, and functional medicine communities. This may differ from standard clinical guidelines. Not medical advice — always discuss with your provider.

General guidelines only. Requirements vary by lab and individual. Confirm with your provider before adjusting any medications.

Turnaround Time

Varies by lab

Clinical Notes

Popular with functional medicine; at-home collection kit available

ICD-10 Codes

Common diagnosis codes associated with this test. Codes are assigned by your provider.

E24.9Cushing syndrome, unspecified
E27.40Adrenocortical insufficiency, unspecified
E27.49Other adrenocortical insufficiency
R53.83Other fatigue

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Lab Codes

Proprietary codes used by each lab for this test.

Quest Diagnostics
19897(order_code)

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For informational purposes only

This page is for educational and research purposes only and does not constitute medical advice, diagnosis, or treatment recommendations. ICD-10 and CPT codes are assigned by your healthcare provider. Always consult a qualified healthcare provider before ordering any lab test or making decisions about your health.